Zhang Yan,Zhang Suo,Wei Wei,Su Yanli,Ding Jing,Zhao Caiping,Dai Zunxiao,Wang Hui,Development of a 2D-HPLC for simultaneous determination of rhynchophylline， isorhynchophylline， corynoxeine and isocorynoxeine[J].SICHUAN MENTAL HEALTH,2022,35(5):424-428

Development of a 2D-HPLC for simultaneous determination of rhynchophylline， isorhynchophylline， corynoxeine and isocorynoxeine

DOI：10.11886/scjsws20220108001

English keywords:2D-HPLC Rhynchophylline Isorhynchophylline Corynoxeine Isocorynoxeine

Fund projects:西安市科技计划项目（项目名称：西安市药学（精神卫生）重点实验室，项目编号：201805051ZD2CG35）；西安市卫生健康委员会/中医药科研项目（项目名称：清幻颗粒抗抑郁药效成分五羟色胺通路作用机制研究，项目编号：SZJ202203）；西安市卫生健康委员会/卫生科研人才项目（项目名称：清幻灵对精神障碍认知功能的改善作用及机制研究，项目编号：J201901004）

Author Name	Affiliation	Postcode
Zhang Yan	Xi'an Mental Health Center， Xi'an 710100， China Xi'an Key Laboratory of Pharmacy （Mental Health）， Xi'an 710100， China	710100
Zhang Suo	Xi'an Mental Health Center， Xi'an 710100， China Xi'an Key Laboratory of Pharmacy （Mental Health）， Xi'an 710100， China	710100
Wei Wei	Xi'an Mental Health Center， Xi'an 710100， China Xi'an Key Laboratory of Pharmacy （Mental Health）， Xi'an 710100， China	710100
Su Yanli^*	Xi'an Mental Health Center， Xi'an 710100， China Xi'an Key Laboratory of Pharmacy （Mental Health）， Xi'an 710100， China	710100
Ding Jing	Xi'an Mental Health Center， Xi'an 710100， China Xi'an Key Laboratory of Pharmacy （Mental Health）， Xi'an 710100， China	710100
Zhao Caiping	Xi'an Mental Health Center， Xi'an 710100， China Xi'an Key Laboratory of Pharmacy （Mental Health）， Xi'an 710100， China	710100
Dai Zunxiao	Xi'an Mental Health Center， Xi'an 710100， China Xi'an Key Laboratory of Pharmacy （Mental Health）， Xi'an 710100， China	710100
Wang Hui	Xi'an Mental Health Center， Xi'an 710100， China Xi'an Key Laboratory of Pharmacy （Mental Health）， Xi'an 710100， China	710100

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English abstract:

Objective To develop a two-dimensional high-performance liquid chromatography （2D-HPLC） for simultaneous determination of the contents of four kinds of Uncaria alkaloids： rhynchophylline， isorhynchophylline， corynoxeine and isocorynoxeine.Methods The 2D-HPLC apparatus was comprised of a first chromatographic column in version Aston SC2 （3.5 mm×25 mm， 5 μm）， an intermediate column in version Aston SH C₁₈ （3.5 mm×10 mm， 5 μm）， and an analytical column in version Aston SCB （4.6 mm×125 mm， 5 μm）. The mobile phase of the first and second liquid chromatography system were CAA-1 and mixed mobile phase （V _{BPI-1 basic mobile phase}∶ V _{MPI-1 mobile phase} ∶ V _{OPI-1 organic mobile phase} = 45∶14∶41）. The chromatographic parameters included a flow rate of 1.0 mL/min， a column temperature of 40℃， a wavelength of 254 nm， an injection volume of 500 μL and a detection time of 9.5 min.Results The linear ranges of rhynchophylline， isorhynchophylline， corynoxeine and isocorynoxeine were 9.77~10 000.00 ng/mL （r=0.999 6）， 10.74~11 000.00 ng/mL （r=0.999 7）， 10.74~11 000.00 ng/mL （r=0.999 7）， 10.74~11 000.00 ng/mL（r=0.999 6）， respectively. The relative standard deviation （RSD） of precision， stability and repeatability were all less than 5.00%. The accuracy was 95.20%~104.01%， and the recovery rate was 93.63%~101.38%.Conclusion The 2D-HPLC developed for simultaneous determination of four kinds of alkaloids in Uncaria is simple and accurate， which can be used as a new method for quality control of Uncaria.

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