| Zhang Yan,Zhang Suo,Wei Wei,Su Yanli,Ding Jing,Zhao Caiping,Dai Zunxiao,Wang Hui,Development of a 2D-HPLC for simultaneous determination of rhynchophylline， isorhynchophylline， corynoxeine and isocorynoxeine[J].SICHUAN MENTAL HEALTH,2022,35(5):424-428 |
| Development of a 2D-HPLC for simultaneous determination of rhynchophylline， isorhynchophylline， corynoxeine and isocorynoxeine |
DOI：10.11886/scjsws20220108001 |
| English keywords:2D-HPLC Rhynchophylline Isorhynchophylline Corynoxeine Isocorynoxeine |
| Fund projects:西安市科技计划项目（项目名称：西安市药学（精神卫生）重点实验室，项目编号：201805051ZD2CG35）；西安市卫生健康委员会/中医药科研项目（项目名称：清幻颗粒抗抑郁药效成分五羟色胺通路作用机制研究，项目编号：SZJ202203）；西安市卫生健康委员会/卫生科研人才项目（项目名称：清幻灵对精神障碍认知功能的改善作用及机制研究，项目编号：J201901004） |
| Author Name | Affiliation | Postcode | | Zhang Yan | Xi'an Mental Health Center, Xi'an 710100, China
Xi'an Key Laboratory of Pharmacy （Mental Health）, Xi'an 710100, China | 710100 | | Zhang Suo | Xi'an Mental Health Center, Xi'an 710100, China
Xi'an Key Laboratory of Pharmacy （Mental Health）, Xi'an 710100, China | 710100 | | Wei Wei | Xi'an Mental Health Center, Xi'an 710100, China
Xi'an Key Laboratory of Pharmacy （Mental Health）, Xi'an 710100, China | 710100 | | Su Yanli* | Xi'an Mental Health Center, Xi'an 710100, China
Xi'an Key Laboratory of Pharmacy （Mental Health）, Xi'an 710100, China | 710100 | | Ding Jing | Xi'an Mental Health Center, Xi'an 710100, China
Xi'an Key Laboratory of Pharmacy （Mental Health）, Xi'an 710100, China | 710100 | | Zhao Caiping | Xi'an Mental Health Center, Xi'an 710100, China
Xi'an Key Laboratory of Pharmacy （Mental Health）, Xi'an 710100, China | 710100 | | Dai Zunxiao | Xi'an Mental Health Center, Xi'an 710100, China
Xi'an Key Laboratory of Pharmacy （Mental Health）, Xi'an 710100, China | 710100 | | Wang Hui | Xi'an Mental Health Center, Xi'an 710100, China
Xi'an Key Laboratory of Pharmacy （Mental Health）, Xi'an 710100, China | 710100 |
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| Objective To develop a two-dimensional high-performance liquid chromatography （2D-HPLC） for simultaneous determination of the contents of four kinds of Uncaria alkaloids： rhynchophylline， isorhynchophylline， corynoxeine and isocorynoxeine.Methods The 2D-HPLC apparatus was comprised of a first chromatographic column in version Aston SC2 （3.5 mm×25 mm， 5 μm）， an intermediate column in version Aston SH C18 （3.5 mm×10 mm， 5 μm）， and an analytical column in version Aston SCB （4.6 mm×125 mm， 5 μm）. The mobile phase of the first and second liquid chromatography system were CAA-1 and mixed mobile phase （V BPI-1 basic mobile phase ∶ V MPI-1 mobile phase ∶ V OPI-1 organic mobile phase = 45∶14∶41）. The chromatographic parameters included a flow rate of 1.0 mL/min， a column temperature of 40℃， a wavelength of 254 nm， an injection volume of 500 μL and a detection time of 9.5 min.Results The linear ranges of rhynchophylline， isorhynchophylline， corynoxeine and isocorynoxeine were 9.77~10 000.00 ng/mL （r=0.999 6）， 10.74~11 000.00 ng/mL （r=0.999 7）， 10.74~11 000.00 ng/mL （r=0.999 7）， 10.74~11 000.00 ng/mL（r=0.999 6）， respectively. The relative standard deviation （RSD） of precision， stability and repeatability were all less than 5.00%. The accuracy was 95.20%~104.01%， and the recovery rate was 93.63%~101.38%.Conclusion The 2D-HPLC developed for simultaneous determination of four kinds of alkaloids in Uncaria is simple and accurate， which can be used as a new method for quality control of Uncaria. |
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